Akademik Veri Yönetim Sistemi
Phytochemical Society of Europe - Trends in Natural Products Research: A PSE Young Scientists’ Meeting, Paris, Fransa, 28 - 30 Haziran 2023, (Yayınlanmadı)
Vitex agnus-castus L. also known as chaste tree and monk’s pepper, belongs to the family Lamiaceae [1]. The genus Vitex includes 214 species in all over the world and only one species, V. agnus-castus found naturally in the Türkiye [2]. The plant is famous for its use in female reproduction system conditions such as mastalgia, menopausal disorders, pre-menstrual syndrome and fertility disorders in women. The polyphenols are one of the main component groups of the plant; while casticin, apigenin, vitexin, kaempferol, isovitexin, luteolin, orientin, penduletin are the primary flavonoids [1,3]. The present study was undertaken to determine phenolic compounds of different parts of the V. agnus-castus which collected from Balıkesir, Türkiye. Phenolic compounds of dried fruit, flower and leaf hydrooalcholic extract’s were determined by LC-HRMS and the cytotoxic effect was evaluated in human breast adenocarcinoma (MCF-7) and human alveolar adenocarcinoma (A549) cell lines using by MTT assay. Appropriate with the data in the literature, the major phenolic compounds of the fruit, flower and leaf extracts were detected as chlorogenic acid, fumaric acid, orientin, casticin, apigenin-7-glucoside and luteolin-7-glucoside. While there are a lot of studies that evaluated the cytotoxic effect of V. agnus-castus, they mainly focused on the fruits or the aerial parts of the plant. To the best of our knowledge, this study is the first to evaluate the cytotoxicity of extracts of flowers. Cytotoxicity test results show that MCF-7 cells are more sensitive than A549 cell line. For both cell lines, fruit extract appears to be more toxic than leaf and flower extracts (p<0.05). While flower extract shows good cytotoxicity in MCF-7 cells with IC50 62.12 µg/mL, its cytotoxicity in A549 cells was very low, at 100 µg/mL the death ratio was 26.7%.