Development of sol-gel phenyl/methyl/poly (dimethylsiloxane) sorbent coating for fabric phase sorptive extraction and its application in monitoring human exposure to selected polycyclic aromatic hydrocarbons using high performance liquid chromatography-fluorescence detection

GAZİOĞLU I., Kabir A., Zengin O. S., Tekkeli E. K., Furton K. G., Tartaglia A., ...More

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, vol.1163, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1163
  • Publication Date: 2021
  • Doi Number: 10.1016/j.jchromb.2020.122520
  • Journal Name: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Polycyclic aromatic hydrocarbons, Fabric phase sorptive extraction, HPLC, Fluorescence detection, Human exposure, Plasma analysis, ARRAY DETECTION METHOD, WHOLE-BLOOD, PLASMA, DRUGS
  • Bezmialem Vakıf University Affiliated: Yes

Abstract

Following the convenient, yet very powerful pathway to create designer extraction sorbent using sol-gel chemistry, a novel sol-gel phenyl/methyl/poly(dimethylsiloxane) sorbent coating was created on polyester fabric substrate for fabric phase sorptive extraction (FPSE) and was subsequently applied to monitor human exposure to selected polycyclic aromatic hydrocarbons (PAHs) including pyrene, chrysene, and benzo[a]pyrene in plasma samples obtained from tobacco smoker volunteers using high performance liquid chromatography fluorescence detector (HPLC-FLD). A rapid FPSE-HPLC-FLD method was developed that adequately resolved the PAHs chromatographically, after their successful extraction from human plasma using fabric phase absorption extraction (FPSE) and subsequently analysed in the liquid chromatographic system by means of an analytical column (InterSustain C-18 column 150 x 4.6 mm, 5 mu m) using acetonitrile (ACN) and water as mobile phases in gradient elution mode. With the optimized conditions, the retention times were found to be 6.168, 7.214, and 10.404 min for pyrene, chrysene, and benzo[a]pyrene, respectively. The total chromatographic runtime was limited to 12.5 min.