Antigenotoxic and antioxidant potentials of newly derivatized compound naringenin-oxime relative to naringenin on human mononuclear cells

Kocyigit A. , Koyuncu I., Taskin A., Dikilitas M., Bahadori F. , Turkkan B.

DRUG AND CHEMICAL TOXICOLOGY, vol.39, no.1, pp.66-73, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.3109/01480545.2015.1026973
  • Page Numbers: pp.66-73
  • Keywords: Comet assay, naringenin, naringenin oxime, natural antioxidant, total antioxidant status, total oxidative status, NF-KAPPA-B, DNA-DAMAGE, OXIDATIVE STRESS, DIETARY FLAVONOIDS, PHENOXYL RADICALS, STRAND BREAKS, BASE DAMAGE, REPAIR, APOPTOSIS, EXTRACTS


We investigated antigenotoxic and antioxidative effects of newly derivatized compound naringenin-oxime (NG-Ox) compared to its mother compound naringenin (NG) against oxidative damage induced by hydrogen peroxide (HP) in human peripheral blood mononuclear cells (PBMC). Antigenotoxic activity was assessed using alkaline single cell gel electrophoresis assay (comet assay). Oxidative status was evaluated by measurement of total antioxidant status, total oxidant status and lipid hydroperoxide levels in the cells. Oxidative stress index was also calculated. Both NG and NG-Ox show a protective effect against HP-induced oxidative damage on PBMC and are able to reduce oxidative stress. The percentage of antigenotoxic and antioxidant potential progressively increased in a dose-dependent manner. However, these activities were found to be more significant in NG-Ox-treated cells than in NG-treated cells. Taken together, these observations provide evidences indicating that both NG and NG-Ox are able to protect cells against oxidative damage and apparently NG-Ox is more effective than NG.