In the present study, neuroprotective effect of Lavandula angustifolia flower aqueous extract in glutamate-induced neurotoxicity in rat pups cerebellar granular cell culture was investigated. The extract at doses of 10 mug ml(-1), 100 mug ml(-1), 1 mg ml(-1) and 10 mg ml(-1) was applied to culture flasks. The extract at doses of 100 mug ml(-1) and 1 mg ml(-1) significantly blocked glutamate-induced neurotoxicity, with the most effective dose being 1 mg ml(-1). On the other hand, 10 mg ml(-1) dose of extract increased the dead cell with respect to glutamate group, despite being found insignificant statistically. As a result, L. angustifolia protected the neurons against glutamate toxicity. (C) 2002 Published by Elsevier Science Ireland Ltd.