Simple high-performance liquid chromatographic method for determination of atropine and obidoxime in a parenteral injection device


Goren A. C. , BILSEL G., BILSEL M., YENISOY-KARAKAS S., KARAKAS D.

JOURNAL OF CHROMATOGRAPHY A, vol.1057, pp.237-239, 2004 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1057
  • Publication Date: 2004
  • Doi Number: 10.1016/j.chroma.2004.09.048
  • Title of Journal : JOURNAL OF CHROMATOGRAPHY A
  • Page Numbers: pp.237-239

Abstract

Atropine and obidoxime in a parenteral injection device are determined by simple HPLC method simultaneously without any pretreatment at 228 nm. The relative standard deviations (R.S.D.) were below 1.6% for the compounds. The correlation coefficient was greater than 0.999 for both compounds in the calibration range. The recoveries at 5 mg/L concentration averaged as 95% for atropine and 102% for obidoxime. The uncertainty of the measurements for atropine and obidoxime was 2.8% and 2.4%, respectively. (C) 2004 Elsevier B.V. All rights reserved.