© 2017, University of Istanbul. All rights reserved.There have been advances in the cell culture models for research and drug studies. The cytotoxicity and permeability of drug molecules and delivery systems are evaluated by cell culture models both in the pharmaceutical industry and in academia. Cell models serve as an important platform to investigate cytotoxicity and permeability studies by reducing the use of animal models. Since 3D cell models mimic in vivo cells better, it plays a significant role in the testing of drugs. This review article emphasizes an overview of cytotoxicity and permeability studies and 3D cell culture model used in pharmaceutical technology.