Akademik Veri Yönetim Sistemi
International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH, Sarajevo, Bosna-Hersek, 18 - 20 Mayıs 2023, (Özet Bildiri)
Breast cancer is a global
public health problem which is seen in more than 2 million women in the world. Due to the limited effectiveness and side effects
of available treatments, search for alternative methods continues in breast
cancer therapy. In particular, modulating the anti-cancer effects of immune
system elements with natural agents has been frequently studied in recent
years. This study aimed to investigate the anti-tumoral effects of a
phytochemical flavone; Chrysin (5,7-dihydroxyflavone) on MCF-7 breast cancer
cells and to determine whether this effect is mediated by immunomodulation of
LPS-induced RAW
264.7 cells. For the MTT cell viability assay, different concentration of
chrysin (12.5-200 µM) were applied to RAW 264.7 and MCF-7 cells in order
to determine non-toxic dose to macrophages and IC50 dose on MCF-7 cells. Raw
264.7 cells were induced by 1 µg/ml LPS and chrysin for 24 hr. After induction,
supernatants of only LPS induced and LPS + chrysin induced macrophage cells
were harvested to analyze the immunomodulatory effect of chrysin. Then MCF-7 cells were also treated with those
supernatants. In order to show how chrysin modulates apoptotic effects of macrophage
cells on MCF-7 cells; acridine orange/ethidium bromide and caspase-3
immunofluorescent staining were performed with fluorescence microscope. According
to the results, IC50 dose of chrysin was found 140 uM which is not cytotoxic to
the Raw 264.7 macrophage cells. In breast cancer cells treated with different supernatants,
the apoptotic activity of chrysin and LPS induced macrophages was found higher
than only LPS induced group due to the more apoptosis detection in fluorescent
images. In conclusion, chrysin has cytotoxic and apoptotic effect on
MCF-7 cells as well as modulation activity on cytotoxic and apoptotic effects
of RAW 264.7 macrophage. However, further studies are required to reveal specific
signaling pathways involved in anti-cancer effect of macrophage cells.