Oral L-arginine protects against cyclosporine-induced hepatotoxicity in rats

Kurus M., Esrefoglu M. , Karabulut A. B. , Sogutlu G., KAYA M., Otlu A.

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, vol.60, pp.411-419, 2008 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 60
  • Publication Date: 2008
  • Doi Number: 10.1016/j.etp.2008.04.007
  • Page Numbers: pp.411-419


Cyclosporine A (CyA) leads to liver injury, probably by causing the production of free radicals and resulting in nitric oxide (NO) deficiency. We evaluated CyA-mediated liver damage histopathologically to determine the possible beneficial effects Of L-arginine (L-Arg). In this study, 7 groups of Sprague-Dawley rats; (1) Control group; (2) 0.9% NaCl group; (3) CyA group: 7.5 mg/kg/day; (4) L-Arg group: 2 g/It/day; (5) L-NAME (N-nitro-L-arginine methyl ester) group: 5mg/100ml/day; (6) CyA+L-Arg group: L-Arg (2 g/It/day) + CyA (7.5mg/kg/day); and (7) CyA + L-NAME group: CyA (7.5mg/kg/day) + L-NAME (5mg/100ml/day) were included. At the end of the treatments, animals were killed and hepatic tissues were treated for morphological (hematoxylin and eosin) and biochemical (NO and malondialdehyde, NIDA) analyses, and serum was processed for biochemical (alanine transaminase (ALT), aspartate transaminase (AST), bilirubin, alkaline phosphatase (ALP) and total protein) study.