An efficient strategy for gene targeting and phenotypic assessment in the Plasmodium yoelii rodent malaria model


Mikolajczak S. A. , Aly A. S. I. , Dumpit R. F. , Vaughan A. M. , Kappe S. H. L.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, cilt.158, ss.213-216, 2008 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 158 Konu: 2
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.molbiopara.2007.12.006
  • Dergi Adı: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
  • Sayfa Sayısı: ss.213-216

Özet

In this report, we describe a cloning procedure for gene replacement by double homologous recombination in Plasmodium yoelii, which requires only one digestion and ligation step. This significantly shortens the time required to complete the production of the targeting vector. Furthermore, for more efficient phenotypic evaluation of the gene knockout parasites, we have also introduced a fluorescent protein cassette into the targeting vector. This allows for a more rapid assessment of parasite growth in all of its developmental stages. In addition, the introduction of the fluorescent marker via the replacement strategy confers the stable integration of the marker. (c) 2007 Elsevier B.V. All rights reserved.