Simultaneous determination of hydrochlorothiazide, amlodipine, and telmisartan with spectrophotometric and HPLC green chemistry applications

Tiris G., Mehmandoust M., Lotfy H. M., Erk N., Joo S., Dragoi E., ...More

CHEMOSPHERE, vol.303, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 303
  • Publication Date: 2022
  • Doi Number: 10.1016/j.chemosphere.2022.135074
  • Journal Name: CHEMOSPHERE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, Greenfile, MEDLINE, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Antihypertensive drugs, Amlodipine, Telmisartan, Hydrochlorothiazide, HPLC, Spectrophotometry, 10 ANTIHYPERTENSIVE DRUGS, UHPLC-MS/MS METHOD, HUMAN PLASMA, LIQUID-CHROMATOGRAPHY, DOSAGE FORM, VALIDATION, QUANTIFICATION, BESYLATE, BULK, MIXTURE
  • Bezmialem Vakıf University Affiliated: Yes

Abstract

For the quantifiable amounts of Telmisartan (TLM) and Hydrochlorothiazide (HYD) in the presence of Amlodipine (AML) in a ternary mixture of synthetic laboratory mixture, a novel, sensitive, quick, and practical reversed-phase high-performance liquid chromatography (RP-HPLC) method was given. In order to separate, a Waters Spherisorb ODS-2 C18 column was used. For HYD, TLM, and AML, these techniques were viable over linearity ranges of 4-12 mu g/mL, 4-25 mu g/mL, and 5-40 mu g/mL, respectively. The mobile phase system was acetonitrile:methanol: phosphate buffer at pH 2.5 (65:5:30 v/v/v), and the flow rate was 1.5 mL/min. Novel spectrophotometric methods were applied for active substances to determine simultaneously. The first method is absorptivity centering using factorized spectrum, and the second method is dual amplitude difference coupled