Rapid quantitation of curcumin in turmeric via NMR and LC-tandem mass spectrometry

Goren A. C., CIKRIKCI S., CERGEL M., BILSEL G.

FOOD CHEMISTRY, vol.113, no.4, pp.1239-1242, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 113 Issue: 4
  • Publication Date: 2009
  • Doi Number: 10.1016/j.foodchem.2008.08.014
  • Journal Name: FOOD CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1239-1242
  • Keywords: Curcumin, Turmeric, Curcuma longa, Quantitative nuclear magnetic resonance, LC-tandem mass spectrometry, Validation, Uncertainty, BISDEMETHOXYCURCUMIN, DEMETHOXYCURCUMIN
  • Bezmialem Vakıf University Affiliated: No

Abstract

A rapid, sensitive and accurate (1)H NMR method has been developed for the quantitation of curcumin isolated from Curcuma longo rhizome (turmeric) extract, the results of which were compared with a validated LC-MS/MS method. The relative standard deviations of the methods were found to be 2.49% and 3.48% for the (1)H NMR and LC-MS/MS methods, respectively. The correlation coefficients were 0.998 for (1)H NMR and 0.995 for LC-MS/MS assay in the calibration range. The recoveries at 5 mg mL(-1) and 50 mu g mL(-1) concentrations averaged to 99-101% for both techniques, respectively. The uncertainty of the measurement Of curcumin via (1)H NMR spectroscopy was determined to he 5.80% while in LC-ESI-MS/MS method was 7.38%. (C) 2008 Elsevier Ltd. All rights reserved.