Folic acid-conjugated amphiphilic copolymers for the enhanced delivery of donepezil: synthesis, characterization and blood-brain barrier permeability in a co-culture model

İğdeli G., Fritzen L., Pietrzik C. U., Temel B.

Journal of Biomaterials Science, Polymer Edition, cilt.36, sa.13, ss.1882-1897, 2025 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 13
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1080/09205063.2025.2486863
  • Dergi Adı: Journal of Biomaterials Science, Polymer Edition
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.1882-1897
  • Anahtar Kelimeler: blood–brain barrier, donepezil, folic acid, polymeric micelles, RAFT polymerization
  • Bezmiâlem Vakıf Üniversitesi Adresli: Evet

Özet

Alzheimer’s disease (AD) is a progressive neurodegenerative disease with limited therapeutic options, largely due to challenges in delivering drugs across the blood–brain barrier (BBB). In this study, we synthesized folic acid (FA) conjugated amphiphilic copolymers via reversible addition-fragmentation chain transfer (RAFT) polymerization to enhance the targeted delivery of donepezil (DZP) to the brain. The copolymers were self-assembled into micelles and extensively characterized for their size, zeta potential, and stability using dynamic light scattering (DLS) and transmission electron microscopy (TEM). The micelles were further evaluated for their ability to cross an in vitro BBB model and their cellular uptake by brain endothelial cells. FA conjugation was employed to exploit the folate receptor-mediated transport mechanism, which has shown potential for improving drug delivery across the BBB. This study demonstrates the feasibility of using FA functionalized micelles as a targeted delivery system, offering potential advancements in the treatment of Alzheimer’s disease.