Evaluation of residual monomer release and toxicity of self-adhesive resin cements


Kurt A., ALTINTAŞ S. H. , KIZILTAS M. V. , TEKKELI S. E. , Guler E. M. , Kocyigit A. , ...More

DENTAL MATERIALS JOURNAL, vol.37, no.1, pp.40-48, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.4012/dmj.2016-380
  • Title of Journal : DENTAL MATERIALS JOURNAL
  • Page Numbers: pp.40-48
  • Keywords: Self-adhesive resin cement, Monomer release, Cytotoxicity, Genotoxicity, ELECTROPHORESIS COMET ASSAY, HUMAN GINGIVAL FIBROBLASTS, SEALANTS IN-VITRO, DENTAL COMPOSITE, DNA-DAMAGE, PULP CELLS, CYTOTOXICITY, COMPONENTS, LYMPHOCYTES, GLUTATHIONE

Abstract

The aim of this study was to evaluate the amount of leached residual monomers from self-adhesive resin cements and evaluate their toxicity in-vitro. A total of 60 disk-shaped specimens (5 mm in diameter and 0.5 mm in thickness) were prepared from each cement (RelyX U200, SpeedCEM, G-Cem) (n=20). Specimens were immersed in artificial saliva and the amount of released monomers [urethane dimethacrylate (UDMA) and triethyleneglycol dimethacrylate (TEGDMA)] was identified. Then, the cytotoxicity and genotoxicity effect on cells were evaluated using the defined amounts of released monomers from cements. The highest monomer release was detected in G-Cem (p<0.05). The highest cytotoxicity value was identified from SpeedCEM (p<0.01) and the highest genotoxicity values were calculated from RelyX U200 (p<0.05). Released UDMA and TEGDMA from self-adhesive resin cements induced cytotoxicity and genotoxicity effect on cells.