Evaluation of residual monomer release and toxicity of self-adhesive resin cements

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Kurt A., ALTINTAŞ S. H., KIZILTAS M. V., TEKKELI S. E., Guler E. M., Kocyigit A., ...More

DENTAL MATERIALS JOURNAL, vol.37, no.1, pp.40-48, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.4012/dmj.2016-380
  • Journal Name: DENTAL MATERIALS JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.40-48
  • Keywords: Self-adhesive resin cement, Monomer release, Cytotoxicity, Genotoxicity, ELECTROPHORESIS COMET ASSAY, HUMAN GINGIVAL FIBROBLASTS, SEALANTS IN-VITRO, DENTAL COMPOSITE, DNA-DAMAGE, PULP CELLS, CYTOTOXICITY, COMPONENTS, LYMPHOCYTES, GLUTATHIONE
  • Bezmialem Vakıf University Affiliated: Yes

Abstract

The aim of this study was to evaluate the amount of leached residual monomers from self-adhesive resin cements and evaluate their toxicity in-vitro. A total of 60 disk-shaped specimens (5 mm in diameter and 0.5 mm in thickness) were prepared from each cement (RelyX U200, SpeedCEM, G-Cem) (n=20). Specimens were immersed in artificial saliva and the amount of released monomers [urethane dimethacrylate (UDMA) and triethyleneglycol dimethacrylate (TEGDMA)] was identified. Then, the cytotoxicity and genotoxicity effect on cells were evaluated using the defined amounts of released monomers from cements. The highest monomer release was detected in G-Cem (p<0.05). The highest cytotoxicity value was identified from SpeedCEM (p<0.01) and the highest genotoxicity values were calculated from RelyX U200 (p<0.05). Released UDMA and TEGDMA from self-adhesive resin cements induced cytotoxicity and genotoxicity effect on cells.