Pyridoxine may protect the cerebellar granular cells against glutamate-induced toxicity

Bueyuekokuroglu M. E. , Gepdiremen A. A. , Tastekin A., Ors R.

INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH, cilt.77, sa.5, ss.336-340, 2007 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 77 Konu: 5
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1024/0300-9831.77.5.336
  • Sayfa Sayıları: ss.336-340


In the present study, the possible protective effect of the pyridoxine against glutamate-induced neurotoxicity in cerebellar granular cell culture of rat pups is investigated for its therapeutic potential. Glutamate (10(-7) M) was administered to cerebellar granular cell cultures that were prepared from one-day-old Sprague-Dawley rats. The neuroprotective effect of pyridoxine was examined. Pyridoxine at the doses of 10(-8), 10(-7), 10(-6), and 10(-5) M was introduced into the culture flasks before inclusion of glutamate. Pyridoxine at the doses of 10(-8) M and 10(-7) M significantly reduced glutamate cytotoxicity. A 10(-7) M dose of pyridoxine proved to be more effective than a 10(-8) M dose. The present study demonstrates that pyridoxine may protect glutamate-induced neurotoxicity. Neuroprotective effect of pyridoxine, at least in part, may result from its anti-glutamatergic activity. Pyridoxine merits further investigation as a therapeutic option in hypoxic-ischemic brain injury.