Effects of Leontice leontopetalum and Bongardia chrysogonum on oxidative stress and neuroprotection in PTZ kindling epilepsy in rats


Erkeç Ö. E. , Arihan O., Kara M., Karataş E., Erten R., Demir H., ...More

CELLULAR AND MOLECULAR BIOLOGY, vol.64, pp.71-77, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 64
  • Publication Date: 2018
  • Doi Number: 10.14715/cmb/2017.64.15.12
  • Title of Journal : CELLULAR AND MOLECULAR BIOLOGY
  • Page Numbers: pp.71-77
  • Keywords: Bcl-2, Brain, Bongardia chrysogonum, Cyclin-B1, GABA(A), kindling model, Leontice leontopetalum, Oxidative stress, Pentylenetetrazol, HIPPOCAMPAL-NEURONS, INDUCED SEIZURES, NIGELLA-SATIVA, ANTIOXIDANT, RECEPTOR, EXPRESSION, EXTRACT, CORTEX, APOPTOSIS, DENSITY

Abstract

We investigated the effects of Leontice leontopetalum and Bongardia chrysogonum on apoptosis, gamma-aminobutyric acid (GABA(A)) receptor positive cell number. cyclin-B1 and bcl-2 levels and oxidative stress in pentylenetetrazol (PTZ.) kindling in rats. Kindling was produced by subconvulsant doses of PTZ treatments in rats. Wistar albino rats were divided into 4 groups; Control, PTZ treated (PTZ), PTZ ;L. leontopetalum extract treated (PTZ FILE) and PTZ ;B. chrysogonum extract treated (PTZ+BCE) groups. Extracts were given a dose (200 mg/kg) 2h before each PTZ, injection. PTZ treatment significantly decreased the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and bc1-2 levels and increased the total oxidant status (TOS), malondialdehyde (MDA), cyclin B1, oxidative stress index (OSI) and number of neurons that expressed GABA(A) receptors when compared to the control. LLE and BCE possessed antioxidant activity in the brain and ameliorated PTZ induced oxidative stress, decreased cyclin-B1, increased bcl-2 levels, and kept the GABA(A) receptor number similar to that of the control despite the PTZ application.