Calmodulin content and in vitro contractility of duodenum from streptozotocin-induced diabetic rats: Effects of insulin therapy and calmodulin antagonism

OZTURK Y., AYDIN S., OZCELIKAY A., Altan V. M., YILDIZOGLUARI N.

EUROPEAN JOURNAL OF PHARMACOLOGY, vol.321, no.1, pp.59-65, 1997 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 321 Issue: 1
  • Publication Date: 1997
  • Doi Number: 10.1016/s0014-2999(96)00928-4
  • Journal Name: EUROPEAN JOURNAL OF PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.59-65
  • Bezmialem Vakıf University Affiliated: No

Abstract

The effects of experimental diabetes and insulin treatment on the decreased reactivity of isolated rat duodenum to KCI and calmidazolium, a specific calmodulin antagonist, were examined. After 8 weeks of streptozotocin diabetes, the contractile effect of KCI and the non-competitive antagonistic effect of calmidazolium against KCI on isolated rat duodenum were decreased. Calmodulin levels, as measured by radioimmunoassay, were also found to be decreased in duodenum from streptozotocin-diabetic rats. Neither impaired reactivity to KCI nor decreased calmodulin levels in diabetic rat duodenum were corrected by treatment with insulin (10 IU/kg for 20 days). Following insulin treatment, there was only a partial correction in the antagonistic effect of calmidazolium as shown by the increase in non-competitive antagonist affinity constant.