Rapid and reliable 25-OH vitamin D2 and 25-OH vitamin D3 measurements by multitasker LC-MS/MS

Goren A. C. , SAHIN G., GUMILCINELI I., BINICI B.

JOURNAL OF CHEMICAL METROLOGY, cilt.12, ss.17-25, 2018 (ESCI İndekslerine Giren Dergi)

  • Cilt numarası: 12 Konu: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.25135/jcm.13.18.02.070
  • Dergi Adı: JOURNAL OF CHEMICAL METROLOGY
  • Sayfa Sayısı: ss.17-25

Özet

The 25-OH Vitamin D2 and 25-OH Vitamin D3 measurement method was validated using a Zivak Multitasker Fully Automated Sample Preparation and Injection System coupled to a MS detector. The current method incorporates all sample preparations, SPE clean up procedures, LC and MS facilities into an all in one system. This system can report patient data within 6 minutes including the SPE and Sample preparation and mass spectrometry analysis time. The correlation coefficient of the developed method was greater than 0.99 for measurands, 25-OH-vitamin Ds, in the calibration range. The measurement uncertainty of the measurements were evaluated and found as 7.7 % and 7.6 % for 25-OH vitamin D2 and 25-OH-vitamin D3, respectively.