Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

Demirci M., Saribas S., Ozer N., TOPRAK S., CAGLAR E., ORTAKOYLU G., ...More

Journal of Infection and Public Health, vol.11, no.5, pp.662-666, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 5
  • Publication Date: 2018
  • Doi Number: 10.1016/j.jiph.2018.02.002
  • Journal Name: Journal of Infection and Public Health
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.662-666
  • Keywords: 85B mRNA, Mycobacterium tuberculosis, RT-qPCR, BACTEC MGIT 960, SPUTUM SPECIMENS, BOVIS BCG, PCR, MARKER, AMPLIFICATION, PURIFICATION, ANTIGENS, COMPLEX, GENE, DNA
  • Bezmialem Vakıf University Affiliated: Yes

Abstract

Background: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TagMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard.