Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection


Demirci M., Saribas S., Ozer N., TOPRAK S., CAGLAR E., ORTAKOYLU G., ...More

Journal of Infection and Public Health, vol.11, no.5, pp.662-666, 2018 (Journal Indexed in SCI Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 5
  • Publication Date: 2018
  • Doi Number: 10.1016/j.jiph.2018.02.002
  • Title of Journal : Journal of Infection and Public Health
  • Page Numbers: pp.662-666

Abstract

Background: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TagMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard.