Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

Demirci M., Saribas S., Ozer N., TOPRAK S., CAGLAR E., ORTAKOYLU G., ...Daha Fazla

Journal of Infection and Public Health, cilt.11, sa.5, ss.662-666, 2018 (SCI Expanded İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Konu: 5
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.jiph.2018.02.002
  • Dergi Adı: Journal of Infection and Public Health
  • Sayfa Sayıları: ss.662-666


Background: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TagMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard.