A rapid and simple method, having high reproducibility and low uncertainty for determination of low concentration of ethanol in water has been developed by using qNMR. Method validation was performed applying CRM and calibration solutions. The correlation coefficient for the analyte in the calibration interval was 1. At a concentration range of 0.5 mg /g to 5 mg/g, the recovery was determined to be 99.71%. The relative uncertainties for ethanol solutions of 0.8123 mg/g and 3.8029 mg/g in water were found to be 0.0053 mg/g and 0.0027 mg/g, respectively.