Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent


Onal C., TEKKELİ Ş. E.

JOURNAL OF RESEARCH IN PHARMACY, cilt.24, sa.5, ss.765-773, 2020 (ESCI İndekslerine Giren Dergi) identifier

  • Cilt numarası: 24 Konu: 5
  • Basım Tarihi: 2020
  • Doi Numarası: 10.35333/jrp.2020.229
  • Dergi Adı: JOURNAL OF RESEARCH IN PHARMACY
  • Sayfa Sayıları: ss.765-773

Özet

A sensitive method is presented for the determination of the ticagrelor (TCG) in human plasma and pharmaceutical preparations using 5-(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl chloride). Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate (pH 10) to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0-200.0 ng mL(-1), with a coefficient of determination of 0.9999 with a limit of detection (LOD) of 0.14 ng mL(-1) and the limit of quantitation (LOQ) of 0.46 ng mL(-1). The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision.