Quantification of Agnuside in Human Plasma with a Novel High-Performance Liquid Chromatographic Method and Pharmacokinetic Study

EGELİ D., TIRIS G., Tekkeli E. K.

JOURNAL OF CHROMATOGRAPHIC SCIENCE, sa.2, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1093/chromsci/bmaf007
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHIC SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Pollution Abstracts, Veterinary Science Database
  • Bezmiâlem Vakıf Üniversitesi Adresli: Evet

Özet

This study presents a combination of High Performance Liquid Chromatography (HPLC) and ultraviolet (UV) detection that provides the quantification of agnuside in human plasma specimens. Reverse-phase chromatographic separation was carried out with C18 column (150 mm x 4.6 mm x 5 mu m), at 25 degrees C with isocratic elution of the mobile phase containing methanol: 0.1% formic acid (35:65 v/v) at 0.6 mL/min flow rate. Experiments were carried out at a wavelength of 258 nm. The retention time of the analyte is 9.70 +/- 0.01 min. The developed technique was validated based on the International Conference on Harmonization guideline. The correlation coefficient of the technique was 0.9915, and the calibration range was 5-125 mu g/mL. The recovery value of the proposed method was found to be 101.4%, and the precision of the method was calculated as 6.35 with the highest RSD% value. A pharmacokinetic study was performed by administering agnuside to a healthy volunteer.