Research Information System
6th ANNUAL MEDICAL STUDENTS’ RESEARCH DAY, İstanbul, Turkey, 14 March 2022, pp.4, (Full Text)
Colorectal cancer it is the third most common cancer in the world. Peganum harmala active ingredient, whose anti-cancer and immunomodulatory effects have been demonstrated in vitro and in vivo studies, is a candidate molecule that can be used as an alternative to conventional cancer treatments. In this study, we will investigate the cytotoxic, apoptotic and genotoxic effects of Peganum harmala on human colon cancer cells. Method: Peganum harmala seed extract (PHSE) has been prepared with 80% MeOH:dH2O solution for two days, MeOH was evaporated by vacuum evaporator and then lyophilized. HT29 colorectal cancer cells and healthy colon epithelial cells (CCD-1072) were incubated with different concentrations of PHSE (200-900 ug/mL) for 24 h. MTT assay was used to measure cytotoxicity of PHSE on each cell line. A fluorescent probe, H2DCF-DA, was used to measure the levels of intracellular reactive oxygen species. Apoptosis was measured using the AO/EB dye using fluorescence microscope. The effect of PHSE on DNA damage was evaluated by alkaline single cell gel electrophoresis (Comet assay method) modified by Singh et al. Results: Peganum harmala seeds extract decrease cell viability and increased intracellular ROS formation in HT29 and CCD cells. The genotoxicity of PHSE on colon cancer increased significantly by concentrationdependently damaging the DNA. 300ug/mL and 500 ug/mL doses of PHSE showed higher genotoxicity than the control, respectively. Also, it has higher genotoxicity in colon cancer compared to healthy cell. Conclusion: The results of this study show that the Peganum harmala extract increased intracellular ROS generation in HT29 and CCD cells. It caused the formation of apoptotic and necrotic cells in the body. The Peganum harmala extract showed higher genotoxicity than the control.