Akademik Veri Yönetim Sistemi
IMPPS 4th International Meeting on Pharmacy & Pharmaceutical Sciences, İstanbul, Türkiye, 18 - 21 Eylül 2014, ss.72, (Özet Bildiri)
Influenza A viruses are enveloped viruses classified in Orthomyxoviridae family. These viruses have a genome composed of
eight single-stranded, negative sense RNA molecules. NS1 protein, which is the subject of this work, is encoded on the eighth
segment, the smallest segment of the viral genome. It is known that one of the most important functions of the NS1 protein
is to block the host interferon defense mechanisms. In this work, it was aimed to detect of the host cellular proteins related
with influenza virus NS1 protein by using yeast two-hybrid method. Human and avian influenza A virus NS1 protein was used
as a bait and assayed for interaction with the proteins encoded by cDNAs of HEK293 cells. Fourteen candidate genes that may
be related with the NS1 protein were determined. Among these genes, the full length sequence of PSMB4 and EEF1G gene
were cloned in pCHA, a mammalian expression vector, to use in research that carried out with mammalian cells in future. The
other candidate genes were named as NKG1 - NKGn. How the specified candidate proteins interact with NS1 protein and how
effect influenza virus replication were not been evaluated within the scope of this work. Elucidation of the action mechanisms
of these proteins require detailed studies on each candidate protein, one by one. Data obtained in this works will be base for
fighting against viral pathogens.