Netrin-1 and its receptor Unc5b as markers of periodontal disease

Gunpinar Ş., Meraci B., Dundar N.

Journal of Periodontal Research, vol.55, no.2, pp.267-276, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 2
  • Publication Date: 2020
  • Doi Number: 10.1111/jre.12712
  • Journal Name: Journal of Periodontal Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, CINAHL, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.267-276
  • Keywords: cellular immunology, gingival crevice fluid, inflammation, macrophages, nonsurgical periodontal therapy, periodontal disease
  • Bezmialem Vakıf University Affiliated: No


© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons LtdObjectives: The aim of this present study was to evaluate the levels of Netrin-1 and Unc5b in periodontal health and disease. Background: Netrin-1, acting via its receptor UNC5b, regulates the inflammatory response and takes apart in bone destructive diseases. Methods: Samples of gingival crevicular fluid (GCF), whole saliva, and serum were taken from systemically healthy, nonsmoking 20 periodontitis, 20 gingivitis patients, and 20 periodontally healthy subjects at baseline and 4 weeks after nonsurgical periodontal treatment (NSPT). Whole-mouth and site-specific clinical periodontal parameters were recorded. Netrin-1 and Unc5b levels were measured by enzyme-linked immunosorbent assay. Data were analyzed by nonparametric tests. Results: Total amount of Netrin-1 in GCF was significantly higher in periodontitis than the others, and the levels were significantly reduced after NSPT. Salivary and serum concentrations of Netrin-1 were significantly different among the study groups (P =.000), and NSPT significantly increased the concentration levels of both salivary and serum Netrin-1 (P <.05). Healthy subjects had significantly lower GCF (P =.001) and conversely, higher salivary and serum levels of Unc5b than the other groups (P =.002). The GCF levels of Unc5b were significantly reduced (P <.01), and conversely, serum concentrations were significantly increased after NSPT (P <.05). GCF Netrin-1 and Unc5b total amounts were positively correlated with clinical parameters (P <.01 and P <.05) whereas salivary Netrin-1 and Unc5b concentrations were negatively correlated with clinical parameters (P <.01 and P <.05). Conclusions: The results of this study indicate that Netrin-1 and its receptor Unc5b may have essential roles in periodontal inflammation and those can be assumed as useful therapeutic agent to control inflammation and periodontal breakdown.