Determination of sulfonamides in milk by ID-LC-MS/MS

Unsal I. A., Tasan M., GOKCEN T., Goren A. C.

JOURNAL OF CHEMICAL METROLOGY, vol.12, no.1, pp.70-78, 2018 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.25135/jcm.13.18.04.081
  • Journal Name: JOURNAL OF CHEMICAL METROLOGY
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.70-78
  • Keywords: Sulfonamides, isotope dilution, LC-MS/MS, method validation, uncertainty estimation, TANDEM MASS-SPECTROMETRY, PERFORMANCE LIQUID-CHROMATOGRAPHY, SOLID-PHASE EXTRACTION, MODIFIED QUECHERS, VETERINARY DRUGS, RESIDUES, VALIDATION, SAMPLES, PRODUCTS, MICROEXTRACTION
  • Bezmialem Vakıf University Affiliated: No

Abstract

In this study, an ID-LC-MS method was developed and validated for the determination of 14 sulfonamides in milk samples. Recovery results were found to be in the range of 91%-114% for milk samples. The relative measurement uncertainty was between 7.5%-12.7%. Validated method was performed on milk samples obtained from market and street vendors. The amount of sulfonamides in the analyzed samples was found to be below the legal limits (Sulfamethazine: 6.46 +/- 0.76 ng/g and sulfisoxazole: 7.3 +/- 0.71 ng/g).