Investigation of serum enzyme activities and oxidative stress markers in preeclampsia: a multiparameter analysis

Selek Ş., Gül A. Z., Atakul N., Meydan S., Sarıkaya U., Köktaşoğlu F., ...More

GINEKOLOGIA POLSKA, vol.0, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 0
  • Publication Date: 2022
  • Journal Name: GINEKOLOGIA POLSKA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Central & Eastern European Academic Source (CEEAS), EMBASE, Gender Studies Database, MEDLINE, Directory of Open Access Journals
  • Bezmialem Vakıf University Affiliated: Yes

Abstract

Objectives: Preeclampsia, a high cause of fetomaternal morbidity-mortality, remains a significant burden affecting 8% of
all pregnancies. Environmental conditions induce disease development leading to endothelial dysfunction in genetically
predisposed women. Our aim is to discuss oxidative stress as a well-established contributing factor to disease progression
with being the first study to show new evidence about serum dehydrogenase enzyme levels (isocitrate, malate, glutamate
dehydrogenase) with oxidative markers (myeloperoxidase, total antioxidant-oxidant status, oxidative stress index).
Material and Methods: Serum parameters were analyzed with photometric method (Abbott ARCHITECT c8000).
Results: The enzyme levels and oxidative markers were significantly higher in patients, supporting the redox imbalance
in preeclampsia. According to ROC analysis, malate dehydrogenase showed an outstanding diagnostic ability with the
highest AUC value of 0.9 and the cut-off value of 51.2 IU/L. Discriminant analysis including malate, isocitrate and glutamate
dehydrogenase had predicted preeclampsia with an overall 87.9% accuracy.
Conclusions: Considering the above results, we propose that the enzyme levels increase with oxidative stress functioning
as antioxidant defense factors. The unique finding of the study is that the serum levels of malate, isocitrate and glutamate
dehydrogenase can be used both separately and combined in the early prediction of preeclampsia. As a novel approach,
we also offer combining serum isocitrate and glutamate dehydrogenase levels with ALT, AST tests to state liver functions
more reliably in patients. Still, larger sample-sized studies investigating enzyme expression levels are required to confirm
the recent findings and to reveal underlying mechanisms.