Investigation of eluted monomers from resin-based root canal sealer by high-performance liquid chromatography analysis

Omurlu H., DENİZ ARISU H., Dalkilic E., TAMER U., TORUL H.

European Journal of Dentistry, vol.10, no.1, pp.92-96, 2016 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.4103/1305-7456.175691
  • Journal Name: European Journal of Dentistry
  • Journal Indexes: Scopus
  • Page Numbers: pp.92-96
  • Keywords: High-performance liquid chromatography, Monomer, Resin, Sealer
  • Bezmialem Vakıf University Affiliated: No


© 2016 European Journal of Dentistry.Objective: The purpose of the current study was to determine the amount of urethane dimethacrylate (UDMA), bisphenol A-glycidyl methacrylate (Bis-GMA), poly (ethylene glycol) dimethacrylate (PEGDMA), bisphenol A ethoxylated dimethacrylate (Bis-EMA), and 2-hydroxyethyl methacrylate (HEMA) eluted from resin-based root canal sealer, epiphany, using high-performance liquid chromatography (HPLC). Materials and Methods: Epiphany was placed into the plastic molds and light-cured with a light emitting diode. After the curing process, each specimen in the first group (n = 12) was immersed in Eppendorf tubes containing a phosphate-buffered saline solution (PBS) and incubated for 45 s. In the second group, each specimen (n = 12) was immersed in Eppendorf tubes containing PBS and incubated for 24 h. Of the specimen extracts, 100 μL were subjected to HPLC. Analysis of data was accomplished with one-way analysis of variance (P < 0.05). Results: All of the samples eluted HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA. A significant difference was determined between the time periods of HEMA, UDMA, PEGDMA, and Bis-EMA (P < 0.05). Conclusion: The results of the current study showed that Epiphany releases HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA in both time periods.