A Liquid Chromatographic Analysis of Deferasirox in Human Breast Milk with Fluorimetric Detection

Onal C., TEKKELİ Ş. E., ASRAM SAĞIROĞLU A.

CHROMATOGRAPHIA, vol.83, no.11, pp.1329-1333, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 83 Issue: 11
  • Publication Date: 2020
  • Doi Number: 10.1007/s10337-020-03953-5
  • Journal Name: CHROMATOGRAPHIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, CAB Abstracts, Chimica, EMBASE, Food Science & Technology Abstracts, Veterinary Science Database, DIALNET
  • Page Numbers: pp.1329-1333
  • Keywords: Defarasirox, Liquid chromatography, Fluorescence detection, Human breast milk, ORAL IRON CHELATOR, 4-BROMOMETHYL-7-METHOXYCOUMARIN, DERIVATIZATION, SURVIVAL, PLASMA
  • Bezmialem Vakıf University Affiliated: Yes

Abstract

To determine deferasirox in human breast milk a novel, sensitive high-performance liquid chromatographic method with fluorescence detection has been developed. Precolumn derivatization process is applied to achieve fluorescent analyte using 4-bromomethyl-7-methoxy coumarin (BrMmC) as a reagent and dibenzo-18-crown-6-ether as catalyst. The method depends on reverse phase chromatography. C(18)column has been used with 150 x 4.6 mm, 5 mu m particle size and the mobile phase consisting of acetonitrile:phosphate buffer (20 mM, pH 3) (70:30,v/v) with a flow rate of 1.0 mL/min. Fluorescence detection at 390 nm is provided with an excitation at 320 nm The linear range is 0.01-1.0 mu g/mL. The mean recovery has been calculated as 100.7% with a relative standard deviation (RSD) value less than 2%. The developed method was successfully applied for the determination of deferasirox in human breast milk samples.