The effects of melatonin in glutamate-induced neurotoxicity of rat cerebellar granular cell culture

Creative Commons License

Gepdiremen A. A., DUZENLI S., HACIMUFTUOGLU A., BULUCU D., SULEYMAN H.

JAPANESE JOURNAL OF PHARMACOLOGY, vol.84, no.4, pp.467-469, 2000 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 84 Issue: 4
  • Publication Date: 2000
  • Doi Number: 10.1254/jjp.84.467
  • Journal Name: JAPANESE JOURNAL OF PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.467-469
  • Bezmialem Vakıf University Affiliated: No

Abstract

In the present study, melatonin was tested in subsequent doses in glutamate induced neurotoxicity in cerebellar granular cell culture of rat pups. Glutamate at 10(-7) M was found to induce neuronal cell death. The dead cell score was 2.75 +/- 0.7 in the control, while it was found to be 35.12 +/- 1.8 in the glutamate-administered group (P<0.0001). Melatonin very potently blocked glutamate neurotoxicity at all doses tested, with 10(-3) M, the highest dose tested, being the most effective. Glutamate may exert a neuroprotective effect by blocking one or more steps of the oxidation cascade in neurons and this effect may be blocked by melatonin.