© 2018, Anka Publishers. All rights reserved.Objective of this study is to investigate the changes of whole genom gene expression levels in rat brain induced by 2,4 GHz Wireless Fidelity Radiofrequency Electromagnetic Field (WiFi RF-EMF). Total RNA was extracted immediately and purified from the rat brain, after 12 hours/day exposed or sham-exposed to a frequency of 2,4 GHz WiFi RF-EMF for 14 days. Roche-Nimblegene-Agilent Microarray System was applied to investigate the changes of gene expression in rat brain. Roche-Nimblegene-Agilent Genespring Software for Microarray Analysis was used in bioenformatic analysis. After 14 days of WiFi RF-EMF exposure, 69 genes in experimental group showed statistical significant down regulation compared with the control group. Ten genes in experimental group showed statistical significant up regulation compared with the control group. In this study, it was detected that WiFi RF-EMF exposure to brain cells resulted with the down and up regulation of transcription level of some important genes.