High Performance Liquid Chromatographic Method For The Determination Of Amlodipine And Perindopril


Lotfy H. M. , Tırıs G. , Erk N.

5th International Turkic World Conference on Chemical Sciences and Technologies, Sakarya, Türkiye, 25 - 29 Ekim 2019, ss.26

  • Basıldığı Şehir: Sakarya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.26

Özet

In the currently work; rapid, sensitive and validated high performance liquid

chromatography (HPLC) method for the simultaneous determination of Amlodipin (ADP) and

Perindopril (PER) in pure forms and pharmaceutical products were presented. Amlodipine

(ADP), 3-O-ethyl 5-O-methyl 2-(2-aminoethoxymethyl)-4-(2-chlorophenyl)-6-methyl-1,4-

dihydropyridine-3,5-dicarboxylate is a vasoselective dihydropyridine calcium antagonist, has

a pharmacokinetic profile that sets it apart from other calcium antagonists (1). Perindopril

(PER) (2S,3aS,7aS)-1-[(2S)-2-[[(2S)-1-ethoxy-1-oxopentan-2-yl]amino]propanoyl]-2,3,3a,4,

5,6,7,7a-octahydroindole-2-carboxylic acid is an angiotensin-converting enzyme inhibitor that

is used in the treatment of hypertension and heart failure. It is also used to reduce the risk of

cardiovascular events in patients with stable ischemic heart disease (2).

Analysis was performed on the Waters Spherisorb® ODS-2 (5μm 150x4.6 mm) column.

Mobile phase system has been consisted of acetonitrile : methanol : posphate buffer (65:5:30

v/v/v). Specimen was enjected to column at 20 μl. Detection wavelength of 220 nm was

preferred for both compounds and flow rate was 1,5 ml/min. Analysis was completed 5

minutes. Column oven temperature was adjusted 30 ºC. The retention time was observed at

1.58 and 2.40 min for PER and ADP. Calibration graphs were constructed in the 10- 40

μg/ml, 5-40 μg/ml concentration of per, adp respectively. The proposed method was

employed with a high degree of precision and accuracy in the determination of all active

ingredients. The method was suitable for the simultaneous determination and it can be applied

in the quality control department in industries, bio-pharmaceutics and bio-equivalence studies.