Ultrastructural clues for glutamate-induced necrosis in parietal and cerebellar neurons


Esrefoglu M. , GEPDIREMEN A. A. , KURUŞ M.

FUNDAMENTAL & CLINICAL PHARMACOLOGY, vol.17, no.3, pp.341-347, 2003 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 3
  • Publication Date: 2003
  • Doi Number: 10.1046/j.1472-8206.2003.00149.x
  • Title of Journal : FUNDAMENTAL & CLINICAL PHARMACOLOGY
  • Page Numbers: pp.341-347

Abstract

Glutamate excitotoxicity has been postulated to underlie the neuronal death that occurs after ischemia. The most sensitive tissues to ischemic injury are hippocampus and cerebellum, whereas cerebrum is more resistant. We studied the glutamate-induced ultrastructural alterations in rat parietal and cerebellar neurons comparatively. We observed that glutamate (45 min, 10(-7) M) causes considerable nuclear, mitochondrial and cytoplasmic changes in both the neuron types. Mitochondrial and nuclear changes were particularly more severe in cerebellar granular, than the ones in parietal neurons. It has been concluded that glutamate induces necrotic changes in both parietal and cerebellar neurons. But cerebellar cortex was found to be more sensitive to glutamate excitotoxicity than cerebral cortex. We suggest that mitochondrial damage is, probably, an important factor in neuron necrosis, which is mediated by glutamate excitotoxicity.