This study investigated the effect of denture cleansers on the surface roughness and Candida albicans adherence of surface sealant agent coupled denture base resins. One hundred and twenty specimens were fabricated from 2 polymethyl methacrylate (PMMA) (Meliodent; Acron MC) and 1 polyamide (Deflex) denture base materials, coated with a sealant agent (Palaseal) and divided into 4 groups (n=10) according to overnight cleaning procedures: distilled water (control), 5% sodium hypochlorite (NaOCl) and two different sodium perborate (Corega; Rapident). The surface roughness values were measured with a profilometer before (R(a)0) and after 90 days immersion in denture cleaners (R(a)1). Specimens were incubated with Candida albicans suspension and Candida colony forming units (CFU) (Cfu/mm) were counted. Significant differences were found, between the R(a)0 and R(a)1 values of 5% NaOCl applied Acron MC, Deflex and also Rapident applied Deflex groups (p<0.05). Denture cleaning procedures had no significant effects on the quantitiy of Candida albicans.