Rapid and simultaneous determination of 25-OH-vitamin D-2 and D-3 in human serum by LC/MS/MS: Validation and uncertainty assessment

Goren A. C. , Bilsel G., Bilsel M.

JOURNAL OF CHEMICAL METROLOGY, cilt.1, sa.1, ss.1-9, 2007 (ESCI İndekslerine Giren Dergi) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1 Konu: 1
  • Basım Tarihi: 2007
  • Dergi Adı: JOURNAL OF CHEMICAL METROLOGY
  • Sayfa Sayıları: ss.1-9

Özet

25-OH Vitamin D-3 and 25-OH Vitamin D-2 were determined by rapid, simple and validated LC/MS/MS method without using SPE and isotopic standard. The relative standard deviations (RSD) were found to be 3.1 and 2.8 % for 25-OH Vitamin D-3 and 25-OH Vitamin D-2, respectively. The correlation coefficient was grater than 0.99 for both analytes in the calibration range. The recoveries at 100 mu g L-1 concentration averaged to 92 % for 25-OH vitamin D-3 and 94 % for 25-OH-vitamin D-2. The uncertainty of the measurements for 25-OH vitamin D-3 and for 25-OH-vitamin D-2 was 7.77 % and 7.24 %, respectively.