Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography


Öztekin N., Baskan S., Kepekci S. E. , Erim F. B. , Topcu G.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.51, ss.439-442, 2010 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 51 Konu: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.jpba.2009.04.012
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Sayfa Sayısı: ss.439-442

Özet

In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia Species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N=3) were 3.269 and 4.518 mu g/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts. (C) 2009 Elsevier B.V. All rights reserved.