LC method for the analysis of paracetamol, caffeine and codeine phosphate in pharmaceutical preparations

Kartal M.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol.26, pp.857-864, 2001 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26
  • Publication Date: 2001
  • Doi Number: 10.1016/s0731-7085(01)00527-1
  • Journal Name: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.857-864
  • Keywords: paracetamol, acetaminophen, caffeine, codeine phosphate, high-performance liquid chromatography, validation, PERFORMANCE LIQUID-CHROMATOGRAPHY, ASCORBIC-ACID, ACETAMINOPHEN, BIOAVAILABILITY, TABLETS
  • Bezmialem Vakıf University Affiliated: No

Abstract

An accurate. simple, reproducible and sensitive method for the determination of paracetamol, caffeine and codeine phosphate has been developed and validated. Paracetamol, caffeine and codeine phosphate were separated using a mu Bondapack C-8 column by isocratic elution with flow rate 1.0 ml/min. The mobile phase composition was 420/20/30/30 (v/v/v/v) 0.01 M KH2PO4, methanol, acetonitrile, isopropyl alcohol and spectrophotometric detection was carried out at 215 nm. The linear range of detection for paracetamol, caffeine and codeine phosphate were between 0.400 and 1500 mug/ml: 0.075 and 90 mug/ml: 0.300 and 30 mug/ml, respectively. The method has been shown to be linear, reproducible, specific, sensitive and rugged. (C) 2001 Elsevier Science B.V. All rights reserved.