Effects of salicylic acid in glutamate- and kainic acid-induced neurotoxicity in cerebellar granular cell culture of rats

Gepdiremen A. A., HACIMUFTUOGLU A., DUZENLI S., OZTAS S., SULEYMAN H.

PHARMACOLOGICAL RESEARCH, vol.42, no.6, pp.547-551, 2000 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 42 Issue: 6
  • Publication Date: 2000
  • Doi Number: 10.1006/phrs.2000.0717
  • Journal Name: PHARMACOLOGICAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.547-551
  • Bezmialem Vakıf University Affiliated: No

Abstract

Glutamate (10(-7) M) and one of its non-NMDA receptor agonists, kainic acid (10(-4) M), were administered to rat cerebellar granular cell cultures, and the neuroprotective role of salicylic acid was examined. Glutamate induced 38.58 +/- 1.45% neuronal cell death while kainic acid induced only 21.4 +/- 2.01% despite being 1000 times more concentrated. The most effective dose for the neuroprotective effect of salicylate in glutamate-induced neurotoxicity was 10(-5) M and it had no protective effect at 10(-7) M. With kainic acid-induced toxicity, 10(-6) M salicylate had no protective effect but 10(-5) M and 10(-4) M salicylic acid were very effective against kainic acid-induced toxicity. As an OH-trapping agent, salicylate had a protective role in NMDA and non-NMDA receptor-activated neuronal cell death. The present study gives some important clues about oxygen free radical generation having an important role in glutamate- and kainic acid-induced neurotoxicity. On the other hand, the neuroprotective effects of salicylic acid in the present study may depend on the pH alterations in salicylic acid solutions. (C) 2000 Academic Press.