Akademik Veri Yönetim Sistemi
Phytochemical Society of Europe - Trends in Natural Products Research: A PSE Young Scientists’ Meeting, Paris, Fransa, 28 - 30 Haziran 2023, (Yayınlanmadı)
Sambucus nigra L. (Adoxaceae) –common names include black elder and European elder– is a
plant native to northern hemisphere which has a high commercial value due to the large amounts
of anthocyanins and other polyphenols found in its fruits [1]. All parts of the plant are sources
of flavonoids while rutin (quercetin-3-O-rutinoside) is the primary flavonoid in all of them. In
addition, fruits contain the anthocyanins cyanidin-3-O-glucoside and cyanidin-3-Osambubioside [2]. The present study was undertaken to determine the phenolic compounds of
leaf, flower and fruit extracts made of S. nigra which were collected from Balıkesir, Türkiye
and to assess the cytotoxicity of the aforementioned extracts using human breast
adenocarcinoma (MCF-7), human alveolar adenocarcinoma (A549) and human brain glioma
(U-118 MG) cell lines.
Hydroalcoholic extracts were obtained and their phenolic contents were determined by LCHRMS. Consistent with the data in the literature, the highest amount of flavonoid in leaf, flower
and fruit extracts was detected as rutin while anthocyanins cyanidin-3-O-glucoside and
cyanidin-3-O-sambubioside were also detected in the fruit extract.
The cytotoxic effect of Sambucus nigra fresh fruit, dried leaf and dried flower extracts were
evaluated in MCF-7, A549 and U-118 MG cell lines using MTT assay. Cytotoxicity test results
show that U-118 MG cells were more sensitive than the other cell lines. In U-188 MG cells the
IC50 values were calculated as 97.06 and 124.6 µg/mL for leaves and flowers, respectively,
while the maximum cell death was 32.9% at 150 µg/mL fruit extracts. In A549 cell line the
maximum cell death rate was 21% at 150 µg/mL leaf extracts and in MCF-7 cell line the
maximum cell death rate was 25.5% at 150 µg/mL leaf extracts. In both A549 and MCF-7 cell
lines, the fruit and flower extracts didn’t show significant cytotoxicity (p<0.05).