Development and validation of a LC method for the analysis of phenolic acids in Turkish salvia species


KAN Y., GOEKBULUT A., Kartal M. , KONUKLUGIL B., YILMAZ G.

CHROMATOGRAPHIA, cilt.66, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

Özet

An accurate, simple, reproducible, and sensitive method for determination of rosmarinic, caffeic, cklorogenic, and gallic acids in 12 Salvio species growing naturally in Anatolia, has been developed and validated. The phenolic acids were separated using a mu Bondapack C-18 column by gradient elution with a flow rate of 1.0 mL min(-1), which was adjusted to deliver firstly o-phosphoric acid 0.085% in water, 0.085% in methanol, and 0.085% in 2-propanol (80:10: 10, v/v/v), then decreased gradually (60:20:20, v/v/v) during 20 min with a flow rate of 1.0 mL min(-1). The samples were monitored at 220 nm for gallic acid and 330 nm for rosmarinic, caffeic, and chlorogenic acids using pkoto-diode array detection. The linear range of detection for gallic, chlorogenic, caffeic, and rosmarinic acids were between 0.051-101.4, 0.207-103.6, 0.100-100, and 0.201-100.5 mu g mL(-1), respectively. The linearity, range, peak purity, selectivity, system performance parameters, precision, accuracy, and robustness had also acceptable values. The developed method was applied to the flower, leaf, stem, and root parts of the Salvia species.