Effects of Intraperitoneal glycerol and flax oil on colonic anastomosis healing process was studied. Thirty female Wistar albino rats were divided into three groups. Each rat underwent segmental colonic resection with single-layer anastomosis. In group-1, the anastomotic line was covered with 9%NaCl, in group-2 with glycerol, and in group-3 with flax oil. The rats were sacrificed on postoperative day 10. Anastomosis burst pressures, tissue hydoxyproline levels and anastomosis histopathological characteristics were evaluated. We observed no statistical differences among all groups in mean bursting pressures (p=0.767). The mean hydroxyprolin level of group 1 was statistically lower than group 2 and 3 (p=0.0001) but no difference between them 3 (p=0.436). Histopathologic evaluation results were not statistically different in all groups. The p values were 0.664 for inflammation, 0.638 for neovascularization, 0.381 for fibroblast ingrowth, and 0.295 for collagen deposition. Glycerol and flax oil are not harmful to the colonic anastomosis healing process. On the contrary, they increase mean tissue levels of hydroxyproline, which is directly related to anastostomotic healing. These liquids securely applicable for colonic anastomosis performed peritoneal cavities.