HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.

Kartal M., ALTUN M. T., KURUCU S.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol.31, no.2, pp.263-269, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 2
  • Publication Date: 2003
  • Doi Number: 10.1016/s0731-7085(02)00568-x
  • Journal Name: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.263-269
  • Keywords: harmol, harmalol, harmine, harmaline, high performance liquid chromatography, validation, PERFORMANCE LIQUID-CHROMATOGRAPHY, BETA-CARBOLINE, ALKALOIDS
  • Bezmialem Vakıf University Affiliated: No

Abstract

A simple and sensitive method for separation and determination of harmol, harmalol, harmine and harmaline has been developed and validated. Harmol, harmalol, harmine and harmaline were separated using a Metasil ODS column by isocratic elution with flow rate 1.5 ml/min. The mobile phase composition was Isopropyl alcohol-Acetonitrile-Water-Formic acid (100:100:300:0.3) (v/v/v/v) and pH adjusted 8.6 with triethylamine. Spectrophotometric detection was carried out at 330 nm. The linear range of detection for harmol, harmalol, harmine and harmaline were between 9.375-250, 30.750-246, 31.250-500 and 31.000-248 mug/ml, respectively. The method described was suitable for the determination of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L. (C) 2002 Elsevier Science B.V. All rights reserved.