Quantification of Ofloxacin in Biological Fluids by High-Performance Liquid Chromatography

Özyazgan S., şenses V., Özüner Z., Akkan A. G.

Journal of Basic and Clinical Physiology and Pharmacology, vol.8, pp.73-80, 1997 (Refereed Journals of Other Institutions) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8
  • Publication Date: 1997
  • Doi Number: 10.1515/jbcpp.1997.8.1-2.73
  • Title of Journal : Journal of Basic and Clinical Physiology and Pharmacology
  • Page Numbers: pp.73-80


A simple HPLC assay was evaluated for analysis of ofloxacin in biological fluids. Mobile phase contained methanol (40%) and phosphate buffer (pH 3.5, 60 mmol/1) and a common C18column was used. Detection was set at 280 nm (UV). Biological fluids (50 μl) were prepared for assay by protein precipitation with acetonitrile (1/1, vol/vol). The assay is linear from 2000 to 10 ng/ml and sensitive to 10 ng/ml. The mean recovery of ofloxacin from serum was 99.4%. The coefficient of variation was < 4.0% for same-day precision and <6.8% for assay-to-assay precision. Because the assay requires only small specimen volumes and minimal sample preparation, this assay provides a simple to use, sensitive and specific method for quantification of ofloxacin in biological fluids. © 2011 by Walter de Gruyter GmbH & Co. All rights reserved.