Study design. This study was designed to compare trace element changes after laparoscopy and laparotomy. Patients, material and methods: Thirty-eight patients who underwent laparoscopy and 35 patients who underwent laparotomy were included in this study. Blood and urine samples were taken preoperatively, at intraoperative 45th min, and postoperative 2nd and 24th hours. Blood hematocrit, plasma CRP, albumin and ceruloplasmin and blood and urine selenium (Se), copper (Cu), zinc (Zn) and ferrum (Fe) concentrations were measured. Results: Plasma Zn concentrations were significantly decreased at postoperative 2nd and 24th hours in both groups whereas the reduction of Fe and albumin levels were significantly more in laparotomy group than laparoscopy group. Plasma CRP levels were significantly higher in both groups at 2nd and 24th hours than preoperative levels. At 24th hour, CRP was increased more in laparotomy group than laparoscopy group. Plasma Se, Cu and ceruloplasmin and urine trace element changes were all insignificant. Preoperative and postoperative hematocrit levels were not changed significantly. The negative correlation was found between CRP levels and Zn and Fe concentrations in both groups. Conclusion: Both in laparoscopy and laparotomy groups, Zn and Fe levels were significantly reduced postoperatively whereas this reduction was more significant in laparotomy patients. Because no change was observed in urine trace elements concentrations and blood hematocrit levels, it was concluded that plasma trace element changes occur due to acute-phase reaction instead of hemodilution.