Research Information System
9. ULUSAL MOLEKÜLER BİYOLOJİ ve BİYOTEKNOLOJİ KONGRESİ, Konya, Turkey, 18 - 20 December 2020
Fibromyalgia
syndrome (FMS) is one of the frequent forms of chronic widespread pain, with a
reported prevalence of 2% to 4% in the adult population. Nervous system
dysfunction is implicated in FMS and neurotransmitters are the targets of a
number of drugs approved for fibromyalgia. However exact underlying mechanisms
of FMS are unknown yet and multiple hypotheses being suggested. Considering the
relation between fibromyalgia and irritable bowel syndrome, altered gut
microbiome could be associated with fibromyalgia. Herein, altered gut
microbiome levels were investigated in patients with FMS compared to healthy
controls. We collected faeces to study the microbiome, from a cohort of 54
patients with FMS and 36 healthy individuals. Extraction of DNA was carried out with commercial kit according to the
manufacturer’s recommendations. Samples were compared using 16S rRNA
gene amplification with specific primers of Bacteroidetes,
Firmicutes, Enterobacteria, Lactobacillus,
Streptococcus and Bifidobacterium by the Real-Time PCR
instrument. According to our results, Bacteroidetes
and Bifidobacteria were increased
statistically significant (p<0.05), Firmicutes
was decreased (p<0.001) in the patient group. No statistically significant
results were found for Enterobacterium,
Streptococcus and Lactobacillus
(p>0.05). When the relationship between bacteria was evaluated,
statistically significant and negative correlation was found between Bacteroidetes and Firmicutes percentage (r =-0.778, p <0.001). A moderately positive
correlation was observed between the percentage of Enterobacter and Bifidobacter
(r = 0.460, p = 0.005). The results suggest that the gut microbiota may play a
role in fibromyalgia.t the gut microbiota may play a role in fibromyalgia. In
sum, it is clear that large-scale further research in larger cohorts will be
efficient in understanding the relationship of gut microbiome and FMS and
evaluating possible treatment options.