Evaluating the effectiveness of anti-tuberculosis treatment by detecting Mycobacterium tuberculosis 85B messenger RNA expression in sputum

Atahan, Ersan; Saribas, Suat; Demirci, Mehmet; Babalık, Aylin; Akkus, Seher; Balıkcı, Ahmet; Satana, Dilek; Ziver, Tevhide; Dinc, HARİKA; Keskin, Melike; Ozbey, Dogukan; Kocak, Banu; Gareayaghi, Nesrin; Kirmusaoglu, Sahra; Tokman, Hrisi; Kocazeybek, Bekir

doi:10.1016/j.jiph.2020.05.016

Evaluating the effectiveness of anti-tuberculosis treatment by detecting Mycobacterium tuberculosis 85B messenger RNA expression in sputum

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Atahan E., Saribas S., Demirci M., Babalık A., Akkus S., Balıkcı A., ...More

Journal of Infection and Public Health, vol.13, pp.1490-1494, 2020 (SCI-Expanded)

Publication Type: Article / Article
Volume: 13
Publication Date: 2020
Doi Number: 10.1016/j.jiph.2020.05.016
Journal Name: Journal of Infection and Public Health
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
Page Numbers: pp.1490-1494
Keywords: 85B mRNA, Mycobacterium tuberculosis, Anti-tuberculosis treatment, PCR, STABILITY, SURROGATE, MARKER, DNA
Bezmialem Vakıf University Affiliated: Yes

Abstract

Background: The antigen 85 complex (85B) is secreted in large quantities from growing mycobacteria and the presence of bacterial mRNA is an indicator of cell viability. The quantitative detection of 85B mRNA expression levels can be used to assess the success of anti-tuberculosis treatment outcomes to detect viable mycobacteria cells. Therefore, we evaluated the levels of 85B mRNA of Mycobacterium tuberculosis strains in patients with pulmonary tuberculosis.