LC-DAD-MS Method for Analysis of Pomiferin and Osajin, Major Isoflavones in Maclura pomifera (Rafin.) Schneider

Kartal M., Abu-Asaker M., DVORSKA M., Orhan I., ZEMLICKA M.

CHROMATOGRAPHIA, vol.69, pp.325-329, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 69
  • Publication Date: 2009
  • Doi Number: 10.1365/s10337-008-0900-3
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.325-329
  • Bezmialem Vakıf University Affiliated: No


Pomiferin and osajin are major bioactive isoflavones found in Maclura pomifera (Rafin.) Schneider (Moraceae). A simple, reproducible, and sensitive method for analysis of pomiferin and osajin in different parts of M. pomifera has been developed and validated. The compounds were separated on a C(8) column by gradient elution at a flow rate at 1.0 mL min(-1). Column eluent was monitored at 280 nm by use of a diode-array detector. Linear range, peak purity, selectivity, system performance, precision, accuracy, and robustness were shown to be acceptable. LC-MS with atmospheric pressure electrospray ionization was used for identification of the compounds; use of positive-ion mode resulted in protonated pseudomolecular ions of the compounds.