Pulse oximetry is a frequently used tool in anesthesia practice. Gives valuable information about arterial oxygen content, tissue perfusion and heart beat rate. In this study we aimed to provide the comparison of peripheral capillary hemoglobin oxygen saturation (SpO(2)) values among every finger of the two hands. Thirty-seven healthy volunteers from operative room stuffs between the ages of 18-30 years were enrolled in the study. They were monitored after 5 min of rest. After their non invasive blood pressure, heart rate, fasting time and body temperature were measured, SpO(2) values were obtained from every finger and each of two hands fingers with the same pulse oximetry. All the SpO(2) values were obtained after at least 1 min of measurement period. A total of 370 SpO(2) measurements from 37 volunteers were obtained. The highest average SpO(2) value was measured from right middle finger (98.2 % +/- 1.2) and it was statistically significant when compared with right little finger and left middle finger. The second highest average SpO(2) value was measured from right thumb and it was statistically significant only when compared with left middle finger (the finger with the lowest average SpO(2) value) (p < 0.05). SpO(2) measurement from the fingers of the both hands with the pulse oximetry, the right middle finger and right thumb have statistically significant higher value when compared with left middle finger in right-hand dominant volunteers. We assume that right middle finger and right thumb have the most accurate value that reflects the arterial oxygen saturation.