Nimodipine improves kainic acid induced neurotoxicity in cerebellar granular cell culture: A double-blind dose-response study


Gepdiremen A. , Sonmez S., Batat I., Esrefoglu M. , Duzenli S., Suleyman H.

FUNDAMENTAL & CLINICAL PHARMACOLOGY, cilt.11, ss.117-120, 1997 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 11 Konu: 2
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1111/j.1472-8206.1997.tb00177.x
  • Dergi Adı: FUNDAMENTAL & CLINICAL PHARMACOLOGY
  • Sayfa Sayıları: ss.117-120

Özet

The neuroprotective role of nimodipine was tested in kainic acid (50 and 100 mu M) induced neurotoxicity in cerebellar granular cell cultures of 4 to 7 day-old rat pups. Nimodipine was applied in 50, 100 and 200 mu M concentrations. Kainate, in either dose, induced cerebellar granular cell death in respect to controls and the results were statistically significant (P = 0.000 for both doses). However, kainic acid in 100 mu M concentration led to higher rates of cell death than 50 mu M (P = 0.017). The neuroprotective role of nimodipine in kainate induced neurotoxicity was dose dependent. Kainate toxicity in 50 mu M concentration was blocked by 50 and 100 mu M nimodipine concentrations (P = 0.006 and P = 0.002, respectively) while 200 mu M nimodipine was found ineffective. The most effective nimodipine dose for 100 mu M kainic acid neurotoxicity was 200 mu M (P = 0.000) while 50 and 100 mu M concentrations of nimodipine were found ineffective. In this study, we have proven the dose-dependent neuroprotective role of nimodipine in kainate induced neurotoxicity in cerebellar granular cell cultures of rat pups.