The effects of ruthenium red, dantrolene and nimodipine, alone or in combination, in NMDA induced neurotoxicity of cerebellar granular cell culture of rats


DUZENLI S., bakuridze k., Gepdiremen A. A.

TOXICOLOGY IN VITRO, cilt.19, ss.589-594, 2005 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 19 Konu: 5
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.tiv.2005.03.007
  • Dergi Adı: TOXICOLOGY IN VITRO
  • Sayfa Sayısı: ss.589-594

Özet

. In the present study, we used the L-type calcium channel blocker nimodipine, the endoplasmic reticulum (ER) calcium release blocker dantrolene and the calcium transport blocker in mitochondria ruthenium red (RuR), in NMDA induced neurotoxicity, to observe the. most suitable combination for neuroprotection in cerebellar granular cell culture of rat pups. In addition we tested the effect of RuR on intact neuronal cells without adding NMDA into the flask, in order to compare the effects. As was expected, NMDA induced neuronal cell death. In NMDA induced neurotoxicity, RuR was the most neuroprotective agent of all three compounds tested, but interestingly, RuR alone was found to be neurotoxic in non-NMDA treated cultures. RuR showed neuroprotectivity in a dose dependent manner in NMDA toxicity. Dantrolene and nimodipine were also found to have neuroprotective properties in NMDA induced cell death in rat cerebellum. On the other hand, the combined application of the compounds was not found as protective as ruthenium red applications alone.