Akademik Veri Yönetim Sistemi
11. TSRM Üreme Sağlığı ve İnfertilite Kongresi, Antalya, Türkiye, 16 - 19 Kasım 2023, ss.1-2, (Özet Bildiri)
OBJECTIVE: It is suggested that abnormal cells or fragments are separated from others by means of self-correction mechanism in embryos. Presence of separate blastomer in blastocysts have been reported to decrease ongoing pregnancies in ART patients. In the current study, we aimed to analyze the morphokinetic behavior of embryos with separate blastomere in blastocyst stage.
MATERIALS-METHODS: METHODS: In this retrospective study, we evaluated the effect of separated blastomer on embryo morphokinetics in 196 blastocysts that were incubated in Time-Lapse Monitoring System (TLM). Due to the possible effects of age, BMI, abnormal semen parameters and ploidy on embryo morphokinetics, we included only euploid embryos of women in the age of <38, BMI ≤25 and with normozoospermic partners. Embryos were assessed according to Gardner’s classification. Embryos were grouped as Top Quality (TQ): Hatched AA, 6AA, 5AA, 4AA, Good Quality (GQ): Hatched AB/BA/BB, 5AB/BA/BB, 4AB/BA/BB, 3AA, Moderate Quality (MQ): 3AB/BA, 2AA. Blastocysts were further grouped according to their presence of separate blastomere. Next Generation Sequencing (NGS) method was perfomed for PGT-A analysis. Mann-Whitney U test was performed for the comparison of morphokinetics. Frequencies were analyzed using Chi-Square test.
RESULTS: Blastocysts with separate blastomer have similar morphokinetics except tPNa, t3 and t5. In separate blastomere group tPNa, t3, t5 were observed earlier than embryos without separate blastomer group (tPNa 7.53±1.43 vs 8.20±1.63 (p=0.036); t3 32.74± 5.79 vs 36.88±4.36 (p=0.001); t5 45.49±4.28 vs 49.39±6.73 (p=0.008)). However, duration of cell cleavage (t2-t8) was longer in separate blastomere group than others, although statistical significance was not obtained (32.85±6.29 vs 30.10±6.95 (p=0.073). No difference was observed in other parameters between two groups (p>0.05). When embryo grade was evaluated, it was observed that the grade of blastocysts with separate blastomere decreased (Frequencies of separate blastomere in blastocysts TQ 0.8%, GQ 15.8%, MQ 40% (p<0.001)).
CONCLUSIONS: Seperate blastomer could affect embryo development. In the current study, we observed that although times to PN apperance, to reach three and five cells were observed earlier in embryos with separate blastomer, the duration of cell cleavage (t2-t8) takes longer. It should be noted that we grouped the blastocysts according to separate blastomere existance in blastocyst stage not in compaction. In our previous studies, we showed that euploid blastocysts with separate blastomere have decreased ongoing pregnancy rates. The main finding of the current study is that the embryos with separate blastomere have decreased quality. Further studies with large sample size are needed to examine what the underlying mechanism and clinical outcomes of separate blostomere existance in embryos are.